Both cellular apoptotic and anti-proliferative effects of Gem were significantly increased after combination with EriB (or Isodon extract). The combination index revealed that Gem and EriB (or Isodon extract) had synergistic anti-proliferative effect. Protein expression regulated by various treatments was analyzed by western blotting. Apoptosis was detected using Annexin V and PI double staining followed by quantitative flow cytometry.
Calcusyn biosoft software#
The combination effect (addition, synergism and antagonism) of various agents was calculated by the Calcusyn software (Biosoft), utilizing the T.C. The anti-proliferation effect was assessed by MTT assay and Ki-67 immunostaining. In addition, the mechanism associated with their effects was also studied. eriocalyx (Isodon) could potentiate the cytotoxic activity of Gem in human pancreatic adenocarcinoma cells. In this study, we further investigated whether EriB or the ethanol extract of I. Our previous studies have demonstrated that eriocalyxin b (EriB), an ent‑kaurane diterpenoid isolated from Isodon eriocalyx (Dunn.) Hara, possesses anti-pancreatic cancer effects, thus acting as a potential therapeutic agent. Therefore, exploring agents that can enhance the anti-cancer activity of Gem would be an attractive strategy. Although gemcitabine (Gem) is the first-line chemotherapeutic drug in the management of pancreatic cancer, the median survival extension is only 1.5 months, indicating unsatisfactory clinical results. Electronic address: cancer, associated with poor prognosis and low survival rate, has been the fourth leading cause of cancer-related death in the US.
7 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.Electronic address: 2 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China. Electronic address: 1 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China. 6 School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.5 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China.4 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, CAS, Yunnan, China.3 Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Hong Kong, China.
0 kommentar(er)
